An important contribution of my work has been the incorporation of mass spectrometry for macromolecules into our local academy, bringing entirely new analytical capabilities for comprehensive high-throughput quantitative proteomic analysis. I have participated in the installation of the first national Biological Mass Spectrometry Service in 2000 located at the Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Ministry of Education and Culture, Uruguay. Until 2006 I have been the operational manager of this service. I have co-founded the Analytical Biochemistry and Proteomics Unit, IIBCE & Institut Pasteur de Montevideo (2007) where I have worked first as Associate Researcher and as Head of the Unit since 2012. Our Unit is a research-oriented core facility that conducts and supports biomedical research based on mass spectrometry and proteomics. Participation in multidisciplinary research teams to provide scientific assistance and access to analytical tools is one of our main objectives. We have also made a long-term effort to provide training and to contribute to local and regional educational programs in this area. The array of strategies and technologies in proteomics is rapidly expanding, thus keeping updated in terms of equipment and trained human resources is a major current challenge.